Growth of Tetraselmis and Nannochloropsis on a laboratory scale

Abstract

Ru’yatin, Rohyani IS, Ali L. 2015. Growth of Tetraselmis and Nannochloropsis on a laboratory scale. Pros Sem Nas Masy Biodiv Indon 1 (2): 296-299. Natural feed has more nutrients than artificial feed and can be a source of essential nutrients on the early stage of organism development. Tetraselmis sp. and Nannochloropsissp. are members of Phytoplankton that are commonly developed as natural feed. As the market demand is growing, supply of natural feed must be available in a large amount, sustainable and in time. It is, therefore, phytoplankton culture is needed to meet the demand. The purpose of this experiment is to examine the growth of Tetraselmis sp. and Nannochloropsissp. at a laboratory scale using KW 21 as growing media in sterilized and salinity adjusted seawater. The observation was done for 18 days for Tetraselmis sp. and 17 days for Nannochloropsissp., based on the change of culture color and phytoplankton’s density. The result showed that Tetraselmis sp. and Nannochloropsissp. have different growth pattern. In stationary phase, Tetraselmis sp. has longer phase than Nannochloropsissp. While the death phase of Nannochloropsissp. is faster than Tetraselmis sp. The density of this phytoplankton is also different. In exponential phase, Nannochloropsissp. grew up to 3,100,000 cells/mL, while Tetraselmis sp. only reached 1,600,000 cells/mLs.