Cellulase activity of isolated fungus from spent straw mushroom substrate

Abstract

Hasanah N, Saskiawan I. 2015. Cellulase activity of isolated fungus from spent straw mushroom substrate. Pros Sem Nas Masy Biodiv Indon 1: 1110-1115. Microbial cellulase plays an important role in the hydrolysis of cellulolytic materials and it is applied at several industrials field. The aim of this experiment was to screen the activity of microbial cellulose of isolated fungus from the spent mushroom substrate. The cellulolytic index of fungus was determined by 0.1% Congo Red staining method with CMC media. The isolates number of JMF 6, JMF 11, and JMF 12 showed the highest cellulolytic index among 20 isolated fungi. The CI value of these isolated number were 0.50, 0.66, and 0.50 respectively. Furthermore, the specific activity of cellulase from selected fungi was determined by measurement of reducing sugar using 3,5-dinitrosalicylic acid (DNS) method. The protein content was determined by the Bradford method. The results showed that the JMF 12 has the highest cellulase activity of 0.77 U/mL and specific activity of 2.78 U/mg. The cellulase activity of the JMF 6 and JMF 11 were 0.48 U/mL and 0.52 U/mL respectively. The specific activity of the JMF 6 and JMF 11 were 1.44 U/mg and 1.83 U/mg respectively.