In vitro spore culture of three variants of the golden chicken fern Cibotium barometz

Abstract

Isnaini Y, Praptosuwiryo TNg. 2015. In vitro spore culture of three variants of the golden chicken fern Cibotium barometz. Pros Sem Nas Masy Biodiv Indon 1: 1649-1653. The golden chicken fern, Cibotium barometz (L.) J. Sm., is an important Indonesian export commodity for both traditional and modern medicine. This trade commodity still relies on harvest from the wild, therefore this species has been included in Appendix II of the Convention on International Trade in Endangered Species (CITES). Morphological and population studies of C. barometz in Sumatra have been conducted in 5 provinces. The results showed that C. barometz of Sumatra consists of three variants which are mainly distinguished by hair color, namely ‘golden yellow’, ‘golden brown’, and ‘pale or white’. The purpose of this study was to compare the rate of growth and development of the gametophytes of three variants of C. barometz in vitro culture. Six genotypes of the three variants were germinated via in vitro spore culture. Spores were sown on a ½ Murashige & Skoog (1/2MS) media with the addition of BAP (0, 2, 4 dan 6 mg/L) and NAA (0; 0.01; 0.03 dan 0.05 mg/L). A ½ MS without BAB and NAA was used for sub-culture. Sporophyte induction used MS, ½ MS, and ¼ MS with the addition of NAA (0; 0.5 dan 1 mg/L), in a factorial arrangement. The first germination occurred 14 days after sowing. For all variants, the highest numbers of rhizoid and filamentous stages were observed on ½ MS media with the addition of 2 mg/L BAP with or without the addition of 0.01 mg/L NAA. For two variants (golden yellow and golden brown) the highest number of sporophytes occurred on ¼ MS without NAA. The highest number of sporophyte formation occurred on one genotype of one variant, namely the ‘golden brown’.