The selection ofligninase enzymeproducing fungi and their ability on palm oil liquid waste degradation

Abstract

Subowo YB. 2015. The selection of ligninase enzyme producing fungi and their ability on palm oil liquid waste degradation. Pros Sem Nas Masy Biodiv Indon 1: 1766-1770. Research on the selection of Basidiomycetes and Ascomycetes fungi that produce ligninase enzymes (Lignin peroxidase, Manganese peroxidase and Laccase) for use in the palm oil liquid waste (POME) degradation has been done. Some types of fungus produce ligninase enzymes, either one or all three of them. These enzymes can break down lignin compounds especially laccase enzyme. The aim was to obtain fungus with a high laccase activity that able to degrade the palm oil liquid waste. The fungi were tested: Aspergillus niger PA2, PenicilliumspR7.5, Pleurotus ostreatus and Lentinus edodes. Palm oil effluent treated with fungal mycelium then incubated for13-30days. The results were P. ostreatus and L. edodes produce laccase enzyme, L. edodes had a higher laccase activity (9.54 units/mL). Penicillium sp.R7.5 produce manganese peroxidase enzyme (40.72 units/mL). P. ostreatus produce three enzymes, LiP; MnP; and Lac. Penicillium sp.R7.5 is able to degrade Poly R-478 as much as 16.46% after incubation for 30 min. Pleurotusos treatus had the highest palm oil waste decolorization result (99,26%) after 30 days incubation. With the addition of CuSO4 and sucrose in the media, P. ostreatus able to reduce color of palm oil liquid waste more quickly, as much as 95.89% after 13 days incubation.