Induksi perakaran dan aklimatisasi tanaman Artocarpus altilis secara in vitro

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SITI NOORROHMAH
MARIA IMELDA

Abstract

Noorrohmah S, Imelda M. 2015. Root induction and acclimatization of in vitro plantlets of Artocarpus altilis. Pros Sem Nas Masy Biodiv Indon 1: 1896-1899. Breadfruit is one of forest plants with a high level of carbohydrate. In a certain area, it becomes an alternative staple food when the main staple foods are scarce. The limited availability of seedling with conventional technology is the main obstacle to the development of breadfruit. Consequently, the multiplication of breadfruit was developed by tissue culture techniques. Tissue culture technology has the ability to produce seedling in a large quantity, with uniform growth rate and in relatively short time. The critical step of propagation by tissue culture techniques is the acclimatization of plantlets. This study aimed to determine the response of explants for root induction with IBA and get proper acclimatization medium for the growth of plantlets breadfruit. This research used Bone cultivar Breadfruit. The study consisted of two steps. The first step was root induction with strength half MS or MS and added IBA (0.25; 0.50; 1.00) mg/L. The second was acclimatization, plantlets were planted which containing cocopeat + carbonized rice hulls + sand + soil (4: 2: 2 :3) and cocopeat + carbonized rice hulls + sand + soil (4: 2: 2: 3) + mycorrhizae was used to plantlets without root. The parameters of in vitro observation were root number and root length while survival rate, root number and root length at acclimatization. The results showed that strength half MS + 0. 50 mg/L IBA increase root number 6.20 and root length 5.04 cm. Acclimatization was performed in the greenhouse with a rate of success 60%. Mycorrhizae increase root number at acclimatization step.

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