Evaluation of quality sexing sperm Friesian Holstein (FH) post thawing

Abstract

Putri RDA, Gunawan M, Kaiin EM. 2015. Evaluation of quality sperm sexing Friesian Holstein(FH) post thawing. Pros Sem Nas Masy Biodiv Indon 1: 2057-2061. Factors that influence the success in artificial insemination (AI) is the quality of the sperm to be inseminated. Frozen sperm storage was done to extend the life of sperm. However, there are any impacts caused by the freezing sperm process. The qualities of sperm are still fresh and will vary on the quality of sperm after thawing. The aim of this study is to determine the quality of FH bull sperm sexing after thawing. Parameters used are the percentage of motility, plasma membrane intact, viability, abnormalities, and morphometry. Sperm motility was analyzed by SpermVision. Abnormalities, morphometry, and viability were analyzed using Eosin-Negrosin and Hoechst dye, and parameters for plasma membrane intact using the three different formulations of Hypo Osmotic Swelling (HOS) Test. The results showed that the highest sperm motility was Sperm X (52.39%). The highest plasma membrane intact was Sperm X (80.95%) and the highest viability was Sperm Y (80.16%). Low sperm abnormality was observed in nonsexing (5. 4%). The conclusion is the quality of sperm X better than Y sperm quality and non-sexing sperm.