Isolation and activity test of IAA and protease producing bacteria from Tual Agricurtural Land, Southeast Maluku

Abstract

Dewi TK, Suryanggono J, Agustiyani D. 2016. Isolation and activity test of IAA and protease producing bacteria from Tual Agricurtural Land, Southeast Maluku. Pros Sem Nas Masy Biodiv Indon 2: 271-276. Indole-3-Acetic Acid producing bacteria may increase the growth and extension of the root and make the root surface becomes more widespread and eventually the plant is able to absorb more nutrients from the soil. Proteases producing bacteria has the ability to break down or eliminate the protein complexes in a polluted environment so that it can play a role in environmental bioremediation. The aim of this study was to isolation and activity test of growth hormone-producing bacteria and proteases producing bacteria from Ohoitel agricultural land, Tual, South East Maluku. Isolation and selection were done by total plate count method on the Triptic Soy Broth and skim milk agar medium. IAA-producing bacteria activity test was done qualitatively and quantitatively. The method used was spectrophotometry at 530 nm and HPLC (High- Performance Liquid Chromatography) with C-18 reverse phase column at 280 nm. Protease enzyme activity measurements were done by the spectrophotometric method at a wavelength of 442 nm. The results showed that isolates 8 Tual able to produce the highest IAA amount 64 ppm. The highest protease enzyme activity showed by TL7C and TL5B isolates amount 635 unit/mL and 551,6 units / mL at 36 hours.