Anatomical structure and histochemical tests of tanin in vegetative and reproductive organs (Rhodomyrtus tomentosa)

##plugins.themes.bootstrap3.article.sidebar##

PDF
Issue
Vol. 9 No. 1 (2023)
Section
Articles

##plugins.themes.bootstrap3.article.main##

IRWANTO
Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lambung Mangkurat. Jl. A. Yani Km 36, Banjarbaru 70714, Kalimantan Selatan, Indonesia
EVI MINTOWATI KUNTORINI ♥
Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lambung Mangkurat. Jl. A. Yani Km 36, Banjarbaru 70714, Kalimantan Selatan, Indonesia
RINI FARIANI
Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lambung Mangkurat. Jl. A. Yani Km 36, Banjarbaru 70714, Kalimantan Selatan, Indonesia

Abstract

Abstract. Irwanto, Kuntorini EM, Fariani R. 2023. Anatomical structure and histochemical tests of tanin in vegetative and reproductive organs (Rhodomyrtus tomentosa). Pros Sem Nas Masy Biodiv Indon 9: 126-132. Karamunting (Rhodomyrtus tomentosa) (Aiton) Hassk has been used as traditional medicine; the phytochemical content of karamunting has many benefits. As a taxonomic indicator, an anatomical study determines where compounds are secreted and accumulated in organs. The aim of this study was to observe the anatomical structure and histochemical tests of tannin compounds in the vegetative and reproductive organs of R. tomentosa. The preparation of anatomical structures using paraffin and safranin staining methods and tannin examination compounds by the histochemical method using 5% FeCl3 reagent in methanol. Observation of the anatomical structure of R. tomentosa leaves consisted of the cuticle, upper and lower epidermis, mesophyll tissue, vascular tissue, midrib parenchyma, collenchyma, and trichomes. The stem's transverse anatomical structure comprises the epidermis, trichomes, secretory spaces, collenchyma, parenchyma, pith, and vascular tissue (xylem and phloem). The anatomical plant structure across the root consists of the epidermis, cortex, pith, secretory space, xylem and phloem vascular tissues. In addition, the transverse anatomical structure of the flower crown is composed of the upper and lower epidermis, parenchyma, collenchyma, secretory spaces, xylem and phloem vascular vessels, and trichomes on both crown surfaces. The transverse anatomical structure of green karamunting fruit consists of the pericarp arranged into three layers: the exocarp/outer layer, mesocarp/middle layer, and endocarp/inner layer, as well as the seeds. The tannin histochemical test results on the vegetative and reproductive organs of R. tomentosa showed a positive reaction to the presence of tannin compounds.

##plugins.themes.bootstrap3.article.details##

References
Arnida A, Hernawati F, Yuwono M. 2010. Kajian farmakognostik simplisia daun karamunting (Rhodomyrtus tomentosa) asal Pelaihari Kalimantan Selatan. Jurnal Ilmiah Berkala Sains dan Terapan Kimia 4 (1): 38-50. [Indonesian]
Dasuki S. 2011. 202 Khasiat Herba. Grup Buku Karangkraf, Selangor.
Dewi GP, Kuntorini EM, Pujawati ED. 2021. Struktur anatomi dan uji histokimia terpenoid dan fenol dua varietas sirih hijau (Piper betle L.). Jurnal Ilmu-ilmu Biologi 17 (2): 1-14. DOI: 10.20527/b.v17i2.3448. [Indonesian]
Dinkelaker B, Hengeler C, Marschner HJBA. 1995. Distribution and function of proteoid roots and other root clusters. Botanica Acta 108 (3): 183-200. DOI: 10.1111/j.1438-8677.1995.tb00850.x.
Evans JR. 1999. Leaf anatomy anables more equal acces to light and CO2 between chloroplasts. New Phytol 143 (1): 93-104. DOI: 10.1046/j.1469-8137.1999.00440.x.
Ferreira P, Mendes C, Reis S, Rodrigues C, Oliveira D. 2011. Morphoanatomy, histochemistry and phytochemistry of Psidium guineense Sw. (Myrtaceae) leaves. J Pharm Res 4 (4): 942-94.
Gogosz AM, Cosmo NL, Bona C, Souza LA. 2010. Morfoanatomia da pla?ntula de Campomanesia xanthocarpa O. Berg. (Myrtaceae). Acta Bot Bras 24 (3): 613-623. DOI: 10.1590/S0102-33062010000300003.
Hamid HA, Mutazah R, Yusoff MM, Karim NAA, Razis AFA. 2017. Comparative analysis of antioxidant and antiproliferative activities of Rhodomyrtus tomentosa extracts prepared with various solvents. Food Chem Toxicol 108: 451-457. DOI: 10.1016/j.fct.2016.10.004.
Hermanto C, Luh N, Indriani P, Hadiati S. 2013. Keragaman dan Kekayaan Buah Tropika Nusantara. IAARD Press, Bogor. [Indonesian]
Kiseleva O, Trofimova L. 2018. Anatomical structure of leaves, stems and roots of hemiparasitic plants Thesium ebracteatum Hayne (Santalacea R. Br.) from the Urals. In Bio web of conferences 11 (00022). EDP Sciences. DOI: 10.1051/bioconf/20181100022.
Kuntorini EM, Sari SG, Fariani R. 2023. The morphoanatomy, histochemistry, and phytochemistry of the leaves and fruits of Rhodomyrtus tomentosa. Biodiversitas 24: 98-105. DOI: 10.13057/biodiv/d240113.
Kuntorini EM, Nugroho LH, Nuringtyas TR. 2019. Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level. Biodiversitas 20 (12): 3619-3625. DOI: 10.13057/biodiv/d201221.
Mohamad J, Maskam MF, Abdulla MA, Wasiman I. 2014. Antioxidant activity of Rhodomyrtus tomentosa (Karamunting) fruits and its effect on lipid profile in induced-cholesterol New Zealand white rabbits. Jurnal Sains Malaysiana 43 (3): 1673-1684.
Mordmuang A, Voravuthikunchai SP. 2015. Rhodomyrtus tomentosa (Aiton) Hassk. leaf extract: An alternative approach for the treatment of staphylococcal bovine mastitis. Research in veterinary science 102: 242-246. DOI: 10.1016/j.rvsc.2015.07.018.
Nugroho H, Purnomo MS, Sumardi I. 2006. Struktur dan Perkembangan Tumbuhan. Penebar Swadaya, Jakarta. [Indonesian]
Retamales HA, Cabello A, Serra MA, Scharaschkin T. 2014. Anatomical study of the flower, fruit and seeds of Myreugenia rufa (Myrtaceae). Boletin Del Nacional de Historia Natural 63: 89-100. DOI: 10.54830/bmnhn.v63.2014.130.
Ribas-Santos, CM, Santos M, Guerra MP. 2016. Development of floral structures of Campomanesia xanthocarpa (Myrtaceae). In II International Workshop on Floral Biology and S-Incompatibility in Fruit Species 1231: 67-74. DOI: 10.17660/ActaHortic.2019.1231.12.
Robil JLM, Tolentino VS. 2015. Histological localization of tannins at different developmental stages of vegetative and reproductive organs in Medinilla magnifica (Melastomataceae). Flora-Morphology, Distribution, Functional Ecology of Plants 217: 82-89. DOI: 10.1016/j.flora.2015.10.003.
Rizki, Selaras GH. Studi Morfologi Organ Vegetatif Karamunting Rhodomyrtus tomentosa (Ait.) Hassk.
Ross S, Speroni G, Souza-Pérez M, Ávila N, Pietro F, González AM, Speranza P. 2021. Stem-cutting anatomy and biochemical responses associated with competence for adventitious root differentiation in Acca sellowiana (Myrtaceae). Trees 35: 1221-1232. DOI: 10.1007/s00468-021-02110-1.
Siahaan MTA, Ambariyanto A, Yulianto B. 2013. Pengaruh pemberian timbal (Pb) dengan konsentrasi berbeda terhadap klorofil, kandungan timbal pada akar dan daun, serta struktur histologi jaringan akar anakan mangrove Rhizophora mucronata. Journal of Marine Research 2 (2): 111-119.
Singh LJ, Misra DR. 2015. Morpho-anatomical diversity of roots of Syzygium cumini Skeels (Myrtaceae): An adaptive strategy under stress ecosystem. Phytomorphology 65 (3): 145-153.
Souza-Moreira TM, Moreira RRD, Sacramento LVS, Pietro RCLR. 2010. Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves. Revista Brasileira de Farmacognosia 20 (1): 48-53. DOI: 10.1590/s0102-695x2010000100011.
Tuladhar A, Ohtsuka S, Nii N. 2014. Formation of exclusive pattern during accumulation of ligno-suberic material in cell wall of Myrtaceae root tissues including epidermis, exodermis, endodermis and polyderm. Plant Root 8: 24-32. DOI: 10.3117/plantroot.8.24.
Wagner WL, Herbst DR, Sohmer SH. 1999. Manual of The Flowering Plants of Hawai'i. Museum Special Publication 83, University of Hawai'i and Bishop Museum Press, Honolulu, HI.
Wu P, Ma G, Li N, Deng Q, Yin Y, Huang R. 2015. Investigation of in vitro and in vivo antioxidant activities of flavonoids rich extract from the berries of Rhodomyrtus tomentosa (Ait.) Hassk. Food chemistry 173: 194-202. DOI: 10.1016/j.foodchem.2014.10.023.
Winotai A, Wright T, Goolsby JA. 2005. Herbivores in Thailand on Rhodomyrtus tomentosa (Myrtaceae), an invasive weed in Florida. Florida Entomologist 88 (1): 104-105. DOI: 10.1653/0015-4040(2005)088[0104:HITORT]2.0.CO;2.