A study on in vitro propagation of Castanopsis argentea

Abstract

Surya MI, Kurnita NI, Setyaningsih L, Ismaini L, Muttaqin Z. 2016. A study on in vitro propagation of Castanopsis argentea. Pros Sem Nas Masy Biodiv Indon 2: 10-15. Saninten (Castanopsis argentea) is a keystone species that has highly potential as a food material. Mostly, the fruits of C. argentea are eaten by animals. It made us difficult to get the natural regeneration. In vitro propagation is an effort to produce considerable amounts of C. argentea. However, the information about in vitro propagation of C. argentea is still very limited. This study was aimed to determine the initiation methods to propagate C. argentea by in vitro propagation. Two methods of sterilization were used to sterilize the explant of seed and buds. Moreover, the explant was planted on modified MS and WPM. The results show that percentage of survival, number of buds and time of germination were found on seed explants sterilized by the first method. The number of callus were found on bud explants sterilized by the second method. Furthermore, planting media were not affected to the germination of seed explants, but affected to growth of bud explants.