Collection method and stomata observation on arrowroot plant using nail polish
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Abstract
Indrayani S, Perdani AY. 2018. Collection method and stomata observation on arrowroot plant using nail polish. Pros Sem
Nas Masy Biodiv Indon 4: 158-162. Arrowroot plant (Maranta arundinacea L.) is an alternative carbohydrate food plant that has not
been widely used and studied. The research aspect is still around the pattern of genetic diversity and its agronomy. While the
physiological aspects, especially cytology has not been done, such as stomata observation. The basic principle of stomata observation is
to take a leaf epidermis and observe it under a microscope. Although it seems simple, there are a number of obstacles encountered
during stomata observation. The obstacles at stomata observation on arrowroot plants are the character of the leaves that easily roll up,
the bottom surface of thin leaves, and there are layers of wax and trichome. This study aims to find the method of removal stomata of
arrowroot plants are easy, cheap, and efficiency to be observed. The material used is the nail polish with some modifications. The
polishing technique is done in three ways: thin, medium, thick combined with long drying. The removal of the wax layers and trichome
on the lower surface of the leaf is done in two ways: direct scratching and adhesive attachment. The results showed that smearing nail
polish thinly with drying more than 1 hour gave the best result. The removal of the wax layers and trichome is more effective with
adhesive apparatus. The best results show a very clear stomata diversity observed in number and size.
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fenologis padi toleran lingkungan kering. Agrotrop 4 (2): 99-103.
Ai NS, Lenak AA. 2014. Penggulungan daun pada tanaman monokotil
saat kekurangan air. Jurnal Bioslogos 4 (2): 48-55.
Campbell NA, Reece JB, Mitchell LG. 1999. Biologi Jilid 2, Edisi ke-2.
Erlangga, Jakarta.
Christine AC, Widiastuti A, Sumardiyono C. 2016. Pengaruh stomata dan
klorofil pada ketahanan beberapa varietas jagung terhadap penyakit
bulai. Jurnal Perlindungan Tanaman Indonesia 20 (2): 89-94.
Fahn A. 1991. Anatomi Tumbuhan. Universitas Gajah Mada Press,
Yogyakarta.
Haryanti S. 2010. Jumlah dan distribusi stomata pada daun beberapa
spesies tanaman dikotil dan monokotil. Buletin Anatomi dan Fisiologi
18 (2): 21-28.
Hidayat E. 1995. Anatomi Tumbuhan Berbiji. ITB Press, Bandung.
Janne J, Palit JJ. 2008. Teknik penghitungan jumlah stomata beberapa
kultivar kelapa. Buletin Teknik Pertanian 13 (1): 9-11.
Lestari EG. 2006. Hubungan antara kerapatan stomata dengan ketahanan
kekeringan pada somaklon padi gajahmungkur, towuti, dan IR 64.
Biodiversitas 7 (1): 44-48.
Marsono Y. 2002. Indeks glisemik umbi-umbian. Makalah Seminar
Nasional Industri Pangan, Perhimpunan Ahli Teknologi Pangan
Indonesia, Surabaya 10-11 Oktober 2002.
Meriko L, Abiza. 2017. Struktur stomata daun beberapa tumbuhan
kantong semar (Nepenthes spp.). Komunikasi pendek. Berita Biologi
16 (3): 325-330.
Mutaqin AZ, Budiono R, Setiawati T, Nurzaman M, Fauzia RS. 2016.
Studi anatomi stomata daun mangga (Mangifera indica) berdasarkan
perbedaan lingkungan. Jurnal Biodjati 1 (1): 13-18.
Steudle E. 2001. The cohesion-tention mechanism and the acquisition of
water by plant roots. Ann Rev Plant Physiol Mol Biol 52: 847-875.
Talutaa HE, Rampea H, Rumondora MJ. Pengukuran panjang dan lebar
pori stomata daun beberapa varietas tanaman kacang tanah (Arachis
hypogaea l.). Jurnal MIPA Unsrat online 6 (2): 1-5.
Utomo JS, Rahmi Y, Astanto K. 2012. Kajian sifat fisikokimia dan
amilografi pati garut dan ganyong. Prosiding Seminar Hasil Penelitian
Tanaman Aneka Kacang dan Umbi 2012. 673-680.