?-Galactosidase enzyme from indigenous Leuconostoc mesenteroides: extraction, partial purification and characterization
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Abstract
Priadi G, Setiyoningrum F, Afaiti F. 2018. ?-Galactosidase enzyme from indigenous Leuconostoc mesenteroides: extraction,
partial purification and characterization. Pros Sem Nas Masy Biodiv Indon 4: 184-189. Dairy industry uses ?-galactosidase in product
development for lactose intolerance. The aim of this study was to determine the characterization of ?-galactosidase partially purified
from indigenous Leuconostoc mesenteroides to temperature and pH. Purification of enzymes using ammonium sulfate with
concentration 40%, 50%, and 60%. Characterization was carried out at 30, 40, 50 and 60 0C, as well as at pH 5, 6 and 7. The results
showed that ?-galactosidase with 50% ammonium sulfate precipitation had the highest specific activity. The optimal work of ?-
galactosidase in hydrolyzing ?-NPG is obtained at temperature 30 0C and pH 7. Ammonium sulfate concentration and temperature
influenced the specific activity of ?-galactosidase enzyme from Leuconostoc mesenteroides.
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References
Abdullah Q, Al-Jibori M, Al-Arrji S. 2014. Extraction purification and
characterization of lipoxygenase from Pleurotus ostreatus. Iraqi J Sci
55 (1): 61-69.
Ahmad R, Jebor MA, Abdulla A, Mahdi RK. 2014. Extraction and
purification of ?-galactosidase from a local isolate of Lactobacillus
acidophilus. Intl J Med Pharmaceut Sci 4 (4): 47-54.
Al-Arrji SB, Al-Hamadi NA. 2017. Extraction, purification and
characterization ?-galactosidase from apricot (Prunus armeniaca
kaisa) fruit for lactose intolerance treatment. Intl J ChemTech Res 10
(6): 919-929.
Beynon R, Bond JS. 2001. Proteolytic enzyme: a practical approach, 2nd
ed. Oxford University Press, Oxford.
Boyer R. 2002. Concept in Biochemistry. Brook Cole: United States.
Busson CS, Foucaud C, Leveau J Y. 1999. Selection of dairy Leuconostoc
isolates for important technological properties. J Dairy Res 66: 245-
256.
Chanalia P, Gandhi D, Attri P, Dhanda S. 2018. Purification and
characterization of ?-galactosidase from probiotik Pediococcus
acidilactici and its use in milk lactose hydrolysis and
galactooligosaccharide synthetys. Bioorg Chem 77: 176-189.
Chakraborti S, Sani RK, Sahoo DK, Benerjee UC, Sobti RC. 2003.
Production and partial characterization of a novel beta galactosidase
from newly isolated Bacillus polymyxa. Scientia Iranica 10: 279-286.
Dake SM, Gupta K. 2012. Isolation of ?-galactosidase from a Yeast sp.
isolated from whey. Intl J Adv Biotechnol Res 6 (3): 425-432.
Duong-Ly KC, Gabelli SB. 2014. Salting out of proteins using ammonium
sulfate precipitation. Methods in Enzymology 541: 85-94.
Fatchiyah, Arumingtyas EL, Widarti S, Rahayu S. 2011. Biologi
Molekuler Prinsip Dasar Analisis. Erlangga, Jakarta.
Gheytanchy E, Heshmati F, Shargh BK, Nowroozi J, Movahedzadeh F.
2010. Study on ?-galactosidase enzyme produced by isolate
Lactobacilli from milk and cheese. African J Microbiol Res 4 (6):
454-458.
Hames BD, Hoper NM. 2000. Biochemstry: The Instant Notes. ed 2.
Springer-Verlag, Hongkong.
Hinz SWA, van den Broek LAM, Beldman G, Vincken JP, Voragen AGJ.
2004. ?-galactosidase from Bifidobacterium adolencentis
DSM290083 prefers ? (1,4)-galactosides over lactose. Appl Microbiol
Biotechnol 66: 276-284.
Husain Q. 2010. Beta galactosidase and their potential application: a
review. Crit Rev Biotechnol 30: 41-62.
Jeong JK, Kwon OS, Lee YM, Oh DB, Lee JM, Kim SH, Kim EH, Lee
TN, Rhee KD, Kang HA. 2009. Characterization of the Streptococcus
pneumonia BgaC protein as a novel surface ?-galactosidase with
specific hydrolysis activity for the Gal ?1-3GlcNAc moiety of
oligosaccharides. J Bacteriol 191: 3011-3023.
Klewicki R. 2007. Formation of gal-sorbitol during lactose hydrolysis
with ? galactosidase. Food Chem 100: 1196-1201.
Lee DH, Kang SG, Suh SG, Byun JK. 2003. Purification and
characterization of a beta-galactosidase from peach (Prunus persica).
Mol Cells 15: 68-74.
Lee JY, Kwak MS, Roh JB, Kim K, Sung MH. 2017. Microbial ?-
galactosidase of Pediococcus pentosaceus ID-7: isolation, cloning,
and molekular characterization. J Microbiol Biotechnol 27 (3): 598-
609.
Lehninger A L. 2004 Principles of Biochemistry. Elsevier Science,
Amhrest.
Maischberger T, Leitner E, Nitisinprasert S, Juajun O, Yamabhai M,
Nguyen TH, Haltrich D. ?-galactosidase from Lactobacillus pentosus:
purification, characterization and formation of galactooligosaccharides.
Biotechnol J 5: 838-847.
Meera N, Theja P, Devi M. 2013. Production and optimization of ?-
galactosidase enzyme using probiotic Yeast spp. Ann Biol Res 4 (12):
62-67.
Natarajan JC, Christobell DJ, Kumar M. 2012. Isolation and
characterization of ?-galactosidase producing Bacillus sp. from dairy
effluent. World Appl Sci J 17 (11): 1466-1474.
Nguyen TH, Splechtina B, Steinbock M, Kneifel W, Lettner HP, Kulbe
KD, Haltrich D. 2006. Purification and characterization of two novel
?-galactosidase from Lactobacillus reuteri. J Agric Food Chem
54:4989-4998.
Picard C, Fioramonti J, Francois A, Robinson T, Neant F, Matuchansky C.
2005. Review article: Bifidobacteria as probiotic agent- physiological
effects and clinical benefits. Aliment Pharmacol Therapeut 22: 495-
512.
Princely S, Basha NS, Kirubaran JJ, Dhanaraju MD. 2013. Biochemical
characterization, partial purification, and production of an
intracellurar beta-galatosidase from Streptococcus thermophilus
grown in whey. Pelagia Research Library 3 (2): 242-251.
Rahman MZ, Maeda M, Kimura Y. 2015. ?-galactosidase from Ginkgo
biloba seeds active against ?-galactosidase-contaijing N-glicans:
purification and characterization. Biosci Biotechnol Biochem 79 (9):
1464-1472.
Setiyoningrum F, Priadi G, Afiati F. 2017. Purifikasi Parsial dan
Karakterisasi Enzim ?-Galaktosidase Isolasi dari Bakteri Asam Laktat
(BAL) Indigenus Lactobacillus farciminis. Prosiding Seminar
Nasional Peternakan Berkelanjutan Nasional 9. Universitas
Padjadjaran, Sumedang 15 November 2017.
Sharma S, Sigh P. 2014. Isolation and characterization of ?-galactosidase
enzyme producing microbe and optimization of its enzyme activity
under different culture condition. Intl J Curr Microbiol Appl Sci 3 (7):
148-155.
Shaukat A, Levitt MD, Taylor BC, Mac Donald R, Shamliyan TA, Kane
RL, Wilt TJ. 2010. Systematic review: Efective management
strategies for lactose intolerance. Ann Internal Med 152 (12): 797-
803.
Singh AK, Sinha S, Singh K. 2009. Study on ?-galactosidase isolation,
purification and optimization of lactose hydrolysis in whey for
production of instant energy drink. Intl J Food Eng 5 (2) art 5:1-10.
Soares I, Tavora Z, Barcelos RP, Baroni S. 2001. Microorganism
produced enzyme in food industry. J Agric Biol Sci 488: 83-84.
Somkuti GA, Dominiecki ME, Steynberg DH. 1998. Permeabilization of
Streptococcus thermophilus and Lactobacillus dekbrueckii subsp.
bulgaricus with ethanol. Current Microbiology 36: 202-206.
Vasiljevic T, Jelen P. 2001. Production of ?-galactosidase for lactose
hydrolysis in milk and products using thermophilic lactic acid
bacteria. J Innov Food Sci Emerg Technol 2: 75-85.
Vinderola CG, Reinheimer JA. 2003. Lactic acid starter and probiotic
bacteria, a comparative “in vitro” study of probiotic characteristic and
biological barriers resistance. J Food Res Intl 36: 895-904
characterization of lipoxygenase from Pleurotus ostreatus. Iraqi J Sci
55 (1): 61-69.
Ahmad R, Jebor MA, Abdulla A, Mahdi RK. 2014. Extraction and
purification of ?-galactosidase from a local isolate of Lactobacillus
acidophilus. Intl J Med Pharmaceut Sci 4 (4): 47-54.
Al-Arrji SB, Al-Hamadi NA. 2017. Extraction, purification and
characterization ?-galactosidase from apricot (Prunus armeniaca
kaisa) fruit for lactose intolerance treatment. Intl J ChemTech Res 10
(6): 919-929.
Beynon R, Bond JS. 2001. Proteolytic enzyme: a practical approach, 2nd
ed. Oxford University Press, Oxford.
Boyer R. 2002. Concept in Biochemistry. Brook Cole: United States.
Busson CS, Foucaud C, Leveau J Y. 1999. Selection of dairy Leuconostoc
isolates for important technological properties. J Dairy Res 66: 245-
256.
Chanalia P, Gandhi D, Attri P, Dhanda S. 2018. Purification and
characterization of ?-galactosidase from probiotik Pediococcus
acidilactici and its use in milk lactose hydrolysis and
galactooligosaccharide synthetys. Bioorg Chem 77: 176-189.
Chakraborti S, Sani RK, Sahoo DK, Benerjee UC, Sobti RC. 2003.
Production and partial characterization of a novel beta galactosidase
from newly isolated Bacillus polymyxa. Scientia Iranica 10: 279-286.
Dake SM, Gupta K. 2012. Isolation of ?-galactosidase from a Yeast sp.
isolated from whey. Intl J Adv Biotechnol Res 6 (3): 425-432.
Duong-Ly KC, Gabelli SB. 2014. Salting out of proteins using ammonium
sulfate precipitation. Methods in Enzymology 541: 85-94.
Fatchiyah, Arumingtyas EL, Widarti S, Rahayu S. 2011. Biologi
Molekuler Prinsip Dasar Analisis. Erlangga, Jakarta.
Gheytanchy E, Heshmati F, Shargh BK, Nowroozi J, Movahedzadeh F.
2010. Study on ?-galactosidase enzyme produced by isolate
Lactobacilli from milk and cheese. African J Microbiol Res 4 (6):
454-458.
Hames BD, Hoper NM. 2000. Biochemstry: The Instant Notes. ed 2.
Springer-Verlag, Hongkong.
Hinz SWA, van den Broek LAM, Beldman G, Vincken JP, Voragen AGJ.
2004. ?-galactosidase from Bifidobacterium adolencentis
DSM290083 prefers ? (1,4)-galactosides over lactose. Appl Microbiol
Biotechnol 66: 276-284.
Husain Q. 2010. Beta galactosidase and their potential application: a
review. Crit Rev Biotechnol 30: 41-62.
Jeong JK, Kwon OS, Lee YM, Oh DB, Lee JM, Kim SH, Kim EH, Lee
TN, Rhee KD, Kang HA. 2009. Characterization of the Streptococcus
pneumonia BgaC protein as a novel surface ?-galactosidase with
specific hydrolysis activity for the Gal ?1-3GlcNAc moiety of
oligosaccharides. J Bacteriol 191: 3011-3023.
Klewicki R. 2007. Formation of gal-sorbitol during lactose hydrolysis
with ? galactosidase. Food Chem 100: 1196-1201.
Lee DH, Kang SG, Suh SG, Byun JK. 2003. Purification and
characterization of a beta-galactosidase from peach (Prunus persica).
Mol Cells 15: 68-74.
Lee JY, Kwak MS, Roh JB, Kim K, Sung MH. 2017. Microbial ?-
galactosidase of Pediococcus pentosaceus ID-7: isolation, cloning,
and molekular characterization. J Microbiol Biotechnol 27 (3): 598-
609.
Lehninger A L. 2004 Principles of Biochemistry. Elsevier Science,
Amhrest.
Maischberger T, Leitner E, Nitisinprasert S, Juajun O, Yamabhai M,
Nguyen TH, Haltrich D. ?-galactosidase from Lactobacillus pentosus:
purification, characterization and formation of galactooligosaccharides.
Biotechnol J 5: 838-847.
Meera N, Theja P, Devi M. 2013. Production and optimization of ?-
galactosidase enzyme using probiotic Yeast spp. Ann Biol Res 4 (12):
62-67.
Natarajan JC, Christobell DJ, Kumar M. 2012. Isolation and
characterization of ?-galactosidase producing Bacillus sp. from dairy
effluent. World Appl Sci J 17 (11): 1466-1474.
Nguyen TH, Splechtina B, Steinbock M, Kneifel W, Lettner HP, Kulbe
KD, Haltrich D. 2006. Purification and characterization of two novel
?-galactosidase from Lactobacillus reuteri. J Agric Food Chem
54:4989-4998.
Picard C, Fioramonti J, Francois A, Robinson T, Neant F, Matuchansky C.
2005. Review article: Bifidobacteria as probiotic agent- physiological
effects and clinical benefits. Aliment Pharmacol Therapeut 22: 495-
512.
Princely S, Basha NS, Kirubaran JJ, Dhanaraju MD. 2013. Biochemical
characterization, partial purification, and production of an
intracellurar beta-galatosidase from Streptococcus thermophilus
grown in whey. Pelagia Research Library 3 (2): 242-251.
Rahman MZ, Maeda M, Kimura Y. 2015. ?-galactosidase from Ginkgo
biloba seeds active against ?-galactosidase-contaijing N-glicans:
purification and characterization. Biosci Biotechnol Biochem 79 (9):
1464-1472.
Setiyoningrum F, Priadi G, Afiati F. 2017. Purifikasi Parsial dan
Karakterisasi Enzim ?-Galaktosidase Isolasi dari Bakteri Asam Laktat
(BAL) Indigenus Lactobacillus farciminis. Prosiding Seminar
Nasional Peternakan Berkelanjutan Nasional 9. Universitas
Padjadjaran, Sumedang 15 November 2017.
Sharma S, Sigh P. 2014. Isolation and characterization of ?-galactosidase
enzyme producing microbe and optimization of its enzyme activity
under different culture condition. Intl J Curr Microbiol Appl Sci 3 (7):
148-155.
Shaukat A, Levitt MD, Taylor BC, Mac Donald R, Shamliyan TA, Kane
RL, Wilt TJ. 2010. Systematic review: Efective management
strategies for lactose intolerance. Ann Internal Med 152 (12): 797-
803.
Singh AK, Sinha S, Singh K. 2009. Study on ?-galactosidase isolation,
purification and optimization of lactose hydrolysis in whey for
production of instant energy drink. Intl J Food Eng 5 (2) art 5:1-10.
Soares I, Tavora Z, Barcelos RP, Baroni S. 2001. Microorganism
produced enzyme in food industry. J Agric Biol Sci 488: 83-84.
Somkuti GA, Dominiecki ME, Steynberg DH. 1998. Permeabilization of
Streptococcus thermophilus and Lactobacillus dekbrueckii subsp.
bulgaricus with ethanol. Current Microbiology 36: 202-206.
Vasiljevic T, Jelen P. 2001. Production of ?-galactosidase for lactose
hydrolysis in milk and products using thermophilic lactic acid
bacteria. J Innov Food Sci Emerg Technol 2: 75-85.
Vinderola CG, Reinheimer JA. 2003. Lactic acid starter and probiotic
bacteria, a comparative “in vitro” study of probiotic characteristic and
biological barriers resistance. J Food Res Intl 36: 895-904