Growth of potato micro shoots cuttings (Solanum tuberosum L. ‘Granola’) in Murashige and Skoog media with the addition of green bean sprouts ekstrak and sucrose

Abstract

Abstract. Muflikhati Z, Pitoyo A, Solichatun. 2022. Growth of potato micro shoots cuttings (Solanum tuberosum L. ‘Granola’) in Murashige and Skoog media with the addition of green bean sprouts ekstrak and sucrose. Pros Sem Nas Masy Biodiv Indon 8: 96-102. Efforts to increase potato production capacity require the fulfillment of large quantities of quality seeds. Potato seedlings are obtained through shoots that grow from potato tubers. Tissue culture allows for the formation of new shoots quickly (micro shoot cuttings). The quality and quantity of micro shoot cuttings produced depend on the accuracy of the media selection. The addition of green bean sprouts extract and sucrose to the media is expected to increase the growth of micro potato cuttings. The aim of this research is to determine the effect of giving various concentrations of green bean sprouts extract and sucrose in MS medium to the growth of micro potato cuttings (Solanum tuberosum L. ‘Granola’). This research used a completely randomized design with variations in the treatment of adding green bean sprout extract five days old and sucrose. The treatments included a ratio of green bean sprouts extract 0; 20; 30; 40 ml/l with variations in sucrose concentration, respectively 0; 20; 30; 40 g/l, so there are 16 treatment combinations with three replications. Explant consists of micro shoots that are the result of cuts from planlets. The treatment was carried out for three months and data collection was performed, which included: the number of shoots, number of leaves, number of roots, plant height, time of shoots appearing, time of roots appearing, root length, wet plant weight, and plant dry weight. Data were analyzed using the ANOVA test at a significance level of 5%. The results of the F test, which had a significant effect, were analyzed using the DMRT test at the 5% significance level. The results showed that applying green bean sprouts extract and sucrose significantly affected the number of shoots, number of leaves, number of roots, plant height, time of shoots appearing, time of roots appearing, root length, wet plant weight, and plant dry weight. The most appropriate treatment concentration was 40 ml/l green bean sprout extract and 40 g/l sucrose. These concentrations can increase the number of leaves and roots, plant height, wet weight, and plant dry weight at the most.