Potential antibacterial, antioxidant, total phenolic and flavonoid content of Trigonachras celebensis extract from Banggai Islands, Central Sulawesi, Indonesia

Abstract

Abstract. Qodrie ENP, Windradi FI, Sahroni D, Praptiwi. 2022. Potential antibacterial, antioxidant, total phenolic and flavonoid content of Trigonachras celebensis extract from Banggai Islands, Central Sulawesi, Indonesia. Pros Sem Nas Masy Biodiv Indon 8: 46-52. Trigonachras celebensis Leenh. (Sapindaceae) is one of the endemic plants of the Banggai Islands. This study aims to determine the antibacterial, antioxidant activity, and the total phenolic and flavonoid content of the methanol extract of the leaves and bark of T. celebensis. Antibacterial activity screening was carried out using the Bioautography-Thin Layer Chromatography method and determination of the Minimum Inhibitory Concentration (MIC) using the Microdilution method. The antioxidant activity test was carried out using the DPPH free radical scavenging method. Determination of total phenolic and flavonoid content was carried out using the Colorimetric method. The results of the study showed that the MIC values of the leaf and bark extract of T. celebensis were 256 g/mL against Staphylococcus aureus and Escherichia coli. The IC₅₀ values of the antioxidant activity of leaves and bark extract were 9.38 g/mL and 12.29 g/mL, respectively, while the AAI values were 3.27 g/mL and 2.51 g/mL. Antioxidant Activity Index (AAI) is positively correlated with total phenolic and flavonoid levels. It could be concluded that leaf and bark ethanol extracts of T. celebensis were more potential to be developed as a source of antioxidants than as antibacterial.