Isolation and identification of fungi associated with Falcataria moluccana gall-rust in Kediri, East Java, Indonesia
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Abstract. Istikorini Y, Cahyani NN, Haneda NF, Shabrina H, Nugroho A, Indriani F, Siregar UJ. 2022. Isolation and identification of fungi associated with Falcataria moluccana gall-rust in Kediri, East Java, Indonesia. Biodiversitas 23: 6171-6179. Gall-rust disease caused by Uromycladium falcatariae is the most important disease of sengon in Indonesia. Plant pathogenic parasitism as a biological control mechanism is usually associated with fungal biocontrol agents. This study aimed to isolate and identify the fungi associated with the sengon gall rust from the Forest Management Unit of Jatirejo, Indonesian State Forest Company, Kediri. Fungi were isolated from the black or gray-green patches of gall-rust samples on Potato Dextrose Agar (PDA) plates. The fungi were identified using macroscopic and microscopic examinations. The molecular identification of fungal DNA was amplified by PCR using a specific internal transcribed spacer primer (ITS1/ITS4). The PCR products were sequenced and compared with the other related sequences in GenBank (NCBI). DNA barcode identification results showed 9 isolates containing of 8 species, namely Fusarium oxysporum, F. solani, F. chlamydosporum, F. circinatum, F. incarnatum, F. verticillioides, Lasiodiplodia theobromae, and Pestalotiopsis palmarum. This result showed that the Fusarium was the most fungi commonly found fungi on the gall's rust teliospore surface.
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References
Altomare C, Perrone G, Zonno MC, Evidente A, Pengue R, Fanti F, Polonelli L. 2000. Biological characterization of fusapyrone and deoxyfusapyrone, two bioactive secondary metabolites of Fusarium semitectum. J. Nat. Prod. 63(8): 1131-1135. DOI: 10.1021/np000023r.
Anggraeni, I. 2009. Penyakit karat tumor pada sengon (Paraserianthes falcataria (L) Nielson). Banyuwangi: Glenmore Press. [Indonesian]
Badan Pusat Statistik. 2018. Statistik Produksi kehutanan 2017. Jakarta: Badan Pusat Statistik.
Badan Pusat Statistik. 2019. Statistik Produksi Kehutanan 2018. Jakarta: Badan Pusat Statistik.
Barnet HL, Hunter BB. 1998. Illustrated Genera of Imperfect Fungi, four editions, The American Phytopathological Scoceity, St. Paul, Minnesota.pp.218.
Baskorowati L. 2014 Budidaya Sengon Unggul (Falcataria moluccana) untuk Pengembangan Hutan Rakyat. Bogor: IPB Press.
Ben H, Huo J, Song C, Yao Y, Gao W, wang W. 2021. Stemphylium lycopersici causing leaf spot of watermelon (Citrullus lanatus) in China. Plant Disease. 102(12). https://doi.org/10.1094/PDIS-05-21-0990-PDN
Berbee ML. 2001. The phylogeny of plant and animal pathogens in the Ascomycota. Physiological and Molecular Plant Pathology. 59(4): 165–187. doi:10.1006/pmpp.2001.0355
Collins RA, Boykin LM, Cruickshank RH, Armstrong KF. 2012. Barcoding’s next top model: an evaluation of nucleotide substitution models for specimen identification. Methods Ecol. Evol. 3: 457-465
Collins RA, Cruickshank RH. 2012. The seven deadly sins of DNA barcoding. Mol Ecol Resour 13 (6): 969–975.
Corryanti, Novitasari D. 2015. Sengon dan Penyakit Karat Tumor. Cepu: Puslitbang Perum Perhutani Cepu.
Dharmaputra OS, Sudirman LI, Ratnaningsih EA. 2018. Mycobiota on banana fruits Lampung cultivar for biological control of Fusarium semitectum. JFI. 14(1): 30-38. DOI: 10.14692/jfi.14.1.30.
Doyle, J. 1991. DNA Protokol for Plant. Part of the NATO ASI Series. Vol 57.
Espinoza JG, Briceño EX, Keith L, Latorre BA. 2008. Cancer and twig dieback in blueberry caused by Pestalotiopsis spp. and a Trucantella sp. in Chile. Plant Dis. 92 (10): 1407-1414. DOI: 10.1094/PDIS-92-10-1407.
Falah S, Achmad, Winara A. 2015. Aktivitas antifungi ekstrak akar mahoni terhadap isolat Botryodiplodia theobromae Pat. penyebab mati pucuk pada bibit jabon. J. Ilmu Teknol. Kayu Tropis 13(1): 1-10. DOI: 10.51850/jitkt.v13i1.363
fruit rot on pre-harvest mango in Malaysia. Plant Pathol Quar 7(2):
Giraldo-Arias J, Celis-Zapata S, Franco-Sierra ND, Arroyave-Toro JJ, Jaramillo-Mazo C, Correa-Álvarez J. Identification of Fusarium cf. verticillioides as the causal agent of Pokka Boheng disease in sugarcane in the department of antioquia, Colombia. Ing. Cienc. 14(28):113–134. https://doi.org/10.17230/ingciencia.14.28.5
Hajibabaei M et al. 2006. A minimalist barcode can identify a specimen whose DNA is degraded. J Compilation Blackwell Publishing. 6: 959-964.
Hajibabaei M, Smith MA, Janzen DH, Rodriguez JJ, Whitfield JB, Hebert PDN. 2006. A minimalist barcode can identify a specimen whose DNA is degraded. Molecular Ecology Notes, 6(4): 959–964. doi:10.1111/j.1471-8286.2006.01470.x
Handayani R. 2016. Akurasi DNA barcode untuk identifikasi jenis Dipterokarpa langka di Hutan Harapan Jambi [tesis]. Bogor: Institut Pertanian Bogor. [Indonesian]
Isniah US, Widodo W. 2015. Eksplorasi fusarium nonpatogen untuk pengendalian penyakit busuk pangkal pada bawang merah. JFI. 11(1): 14-22. DOI: 10.14692/jfi.11.1.14 [Indonesian]
Krisnawati H, Varis E, Kallio M, & Kanninen M. 2011. Paraserianthes falcataria (L.) Nielsen: Ekologi, silvikultur dan produktivitas. In Paraserianthes falcataria (L.) Nielsen: Ekologi, silvikultur dan produktivitas. CIFOR. DOI: 10.17528/cifor/003482. [Indonesian]
Kumar S, Stecher G, Li M, Knyaz C, and Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molec. Biol. Evol. 35:1547–1549
Leslie JL, Summerell BA.2003. A Utilitarian to Fusarium Identification. Plant Dis. 87: 117-128. DOI: 10.1094/PDIS.2003.87.2.117
Lestari P, Rahayu S, Widiyatno. 2013. Dynamics of gall-rust disease on sengon (Falcataria moluccana) in various agroforestry patterns. Procedia Environ. Sci. 17: 167–171. DOI: 10.1016/j.proenv.2013.02.025
Li Y, Ji P. 2015. First report of fruit rot of watermelon caused by Fusarium equiseti in Georgia in the United States. Plant Dis. 99 (9): 1074. DOI: 10.1094/PDIS-10-14-1074-PDN.
Li YG, Song XL, Wang XQ, Zhang H, Tian S, Ji P. 2018. First report of fruit rot of watermelon caused by Fusarium equiseti in China. Plant Dis. 102 (9): 199. DOI: 10.1094/PDIS-01-18-0199-PDN.
Mathivanan N, Murugesen K. 2000. Fusarium clamydosporum, a potent biocontrol agent to groundnut rust, Puccinia arachidis. JPDP. 107 (3): 225-234.
Munirah MS, Azmi AR, Yong SYC, Nur AIMZ (2017) Characterization
Narita V, Arum AL, Isnaeni S, Fawzya NY. 2012. Analisis bioinformatika berbasis web untuk eksplorasi enzim kitosanase berdasarkan kemiripan sekuens. Jurnal Al-Azhar Indonesia Seri Sains Dan Teknologi. 1(4):197–203. [Indonesian]
Nel B, Steinberg C, Labuschagne N, Viljoen A. 2006. The potential of nonpathogenic Fusarium oxysporum and other biological control microorganisms for suppressing fusarium wilt of banana. Plant Pathol. 55: 1217-1223. DOI: 10.1111/j.1365-3059.2006.01344.x.
Nugraha F, Roslim DI, Ardilla YP, Herman. 2014. Analysis of Partial Gene Sequence Ferritin2 on Rice Plants (Oryza sativa L.) Indragiri Hilir, Riau. Biosaintifika. 6(2) :94-103. DOI: 10.15294/biosaintifika.v6i2.3102
Nugroho A, Matra DD, Siregar IZ, Haneda NF, Istikorini Y, Rahmawati R, Amin Y, Siregar UJ. 2021. Early growth evaluation and estimation of heritability in a sengon (Falcataria moluccana) progeny testing at Kediri, East Java, Indonesia. Biodiversitas 22(5): 2728-2736. DOI: 10.13057/biodiv/d220534
Nuroniah HS, Tata HL, Mawazin, Martini E, Dewi S. 2021. Penilaian kesesuaian penanaman spesies lahan gambut non-asli Falcataria moluccana (Miq.) Barneby & Grimes di lahan gambut rewetted. Keberlanjutan 13, 7015. DOI: 10.3390/su13137015 [Indonesian]
of Lasiodiplodia theobromae and L. pseudotheobromae causing
Pampile J, Azevedo JL. 2002. Molecular characterization of endophytic strains of Fusarium verticilioides (Fusarium moniliforme) from maize (Zea mays L.). World J. Microbiol. Biotechnol. 18(5): 391-396. DOI: 10.1023/A:1015507008786.
Putri MA. 2015. Variasi genetik rotan berdasarkan penanda DNA barcode matK, rbcL, dan ITS pada pangkalan data GenBank [skripsi]. Bogor: Institut Pertanian Bogor. [Indonesian]
Rahayu S, Lee SS, Aini, NAS, Shaleh G. 2009. Responses of Falcataria moluccana seedlings of different seed sources to inoculation with Uromycladium tepperianum. Silvae Genet. 58: 62-67. DOI: 10.1515/sg-2009-0008
Robbani A. 2016. Identifikasi Melastoma sp. asal Jambi menggunakan DNA barcoding matK (Maturase K) [skripsi]. Bogor : Institut Pertanian Bogor. [Indonesian]
Roslim DI, Oktavia S, Herman. 2015. Partial DNA Sequences Analysis of Linamarase Gene in Menggalo and Roti Cassavas (Manihot esculenta Crantz.). JDP. 30(2):109–116.
Salvatore MM, Andolfi A, Nicoletti R. 2020. The thin line between pathogenicity and endophytism: The case of Lasiodiplodia theobromae. Agriculture. 10(10): 1–22. DOI: 10.3390/agriculture10100488.
Siregar UJ, Olivia RD. 2012. Genetic diversity of Sengon population (Paraserianthes falcataria(L))in Java community forest based on RAPD marker. JST. 3(2): 1-7. DOI: 10.29244/j-siltrop.3.2.%25p
Sutejo AM, Priyatmojo A, Wibowo A. 2008. Morphological identification of several Fusarium species. JPTI. 14(1): 7-13. DOI: 10.22146/jpti.11870
Urbez-Torres JR, Leavitt GM, Guevara J, Gubler W, Guerrero JC. 2008. Identification and pathogenicity of Lasiodiplodia theobromae and Diplodia seriata, the causal agents of bot cancer disease of grapevines in Mexico. Plant Dis. 92 (4): 519-529. DOI: 10.1094/PDIS-92-4-0519.
Valentini A, Pompanon F, Taberlet P. 2009. DNA Barcoding for Ecologists.Trends Ecol. Evol. (24)2:110-117. DOI: 10.1016/j.tree.2008.09.011
Wahyuno D. 2008. Mengendalikan cendawan karat pada kana dengan mikoparasit. WARTA Penelitian dan Pengembangan Pertanian. 30(2): 13-14. [Indonesian]
Watanabe T. 2002. Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species. Edisi ke 2. New York (US): CRC PRESS.
Widyastuti SM, Harjono, Surya ZA. 2013. Initial infection of Falcataria moluccana leaves and Acacia mangium phyllodes by Uromycladium tepperianum fungi in a laboratory trial. JMHT 19 (3): 187-193. DOI: 10.7226/jtfm.19.3.187
Xiao J, Hu JY, Sun HD, Zhao X, Zhong WT, Duan DZ, Wang L, Wang XL. 2018. Sinopestalotiollides A-D, cytotoxic diphenyl ether derivatives from plant endophytic fungus Pestalotiopsis palmarum. Bioorg. Med. Chem. Lett.. 28 (3): 515-518. DOI: 10.1016/j.bmcl.2017.11.044.
Yusuf SE, Djatnika I, Suhardi. 2014. Collection and characterization of mycoparasite from rust disease on chrysanthemum. J.Hort. 24 (1): 56-64
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