Phenotypic performance of Srikayang's shallot variety M1 by colchicine induction

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ANNISA ADELIA NUR RAHMAWATI
NANDARIYAH
PARJANTO

Abstract

Abstract. Rahmawati ADN, Nandariyah, Parjanto. 2024. Phenotypic performance of Srikayang's shallot variety M1 by colchicine induction. Biodiversitas 25: 1297-1303. Srikayang shallot is one of the shallot varieties from Kulonprogo Yogyakarta, and it has relatively small bulbs. Indonesian consumers prefer shallot, which has dense bulbs, is large (diameter ? 2.5 cm), has a spicy taste, and is fragrant. The efforts to increase shallot's diversity are important. This study aimed to evaluate the performance of Srikayang shallot M1 and evaluate Lethal Doses (LD50) of colchicine. This research was conducted from September to November 2023 at Balecatur Planting Garden, Yogyakarta. Srikayang shallot bulbs were used, and colchicine concentration consisted of 0%, 0.05%, 0.075%, and 0.1%, with a soaking period of 3, 6, and 12 hours. The study was conducted using a randomized block design (RBD). Data were analyzed using descriptive analysis and probit analysis. The colchicine concentration and soaking duration affected the morphological character of Srikayang plants in leaf color, bulb skin color, bulb shape, bulb neck shape, and root tip shape. This study showed that the interaction of colchicine concentration and soaking duration improved variability on plant height, number of leaves, number of saplings, bulb weight in the cluster, main bulb weight, and main bulb diameter. The LD50 of Srikayang shallots was obtained at a colchicine concentration of 0.065%.

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References
Ayu GM, Elimasni, Nurwahyuni I. 2019. Physiological effect of colchicine treatment to garlic (Allium sativum L.) cv. Doulu. IOP Conf Ser: Earth Environ Sci 305 (209): 012073. DOI: 10.1088/1755-1315/305/1/012073.
Billa AT, Lestari SS, Daryono BS, Subiastuti AS. 2022. Bio-charantin effects on phenotypic traits and chromosome number of shallots (Allium cepa L. var ascalonicum Tajuk). Sabrao J Breed Genet 54 (2): 350-358. DOI: 10.54910/sabrao2022.54.2.11.
Cabahug RAM, Khanh HTT, Lim KB, Hwang YJ. 2021. Phenotype and ploidy evaluation of colchicine induced Echeveria ‘Peerless’. Toxicol Environ Health Sci 13 (1): 17-24, DOI: 10.1007/s13530-020-00069-z.
Corneillie S, Storme ND, Acker RV, Fangel JU, Bruyne MD, Rycke RD, Geelen D, Willats WGT,Vanholme B, Boerjan W. 2019. Polyploidy affects plant growth and alters cell wall composition. Plant Physiol 179: 74-87. DOI: 10.1104/pp.18.00967.
Dixit V, Chaudhary BR. 2014. Colchicine-induced tetraploidy in garlic (Allium sativum L.) and its effect on allicin concentration. J Hortic Sci Biotechnol 89 (5): 585-591. DOI: 10.1080/14620316.2014.11513124.
Eng WH, Ho WS. 2019. Polyploidization using colchicine in horticultural plants: a review. Scientia Horticulturae 246: 604-617. DOI: 10.1016/j.scienta.2018.11.010.
Farhadi N, Panahandeh J, Azar AM, Mokhtarzadeh S. 2022. Production of autotetraploid plants by in vitro chromosome engineering in Allium hirtifolium. J Hortic Plant 9 (5): 986-998. DOI: 10.1016/j.hpj.2022.12.013.
Fathurrahman F, Mardaleni, Kristianto A. 2023. Effect of colchicine mutagen on phenotype and genotype of Vigna unguiculata var. sesquipedalis the 7th generation. Biodiversitas 24 (3): 1408-1415. DOI: 10.13057/biodiv/d240310.
Gantait S, Mukherjee E. 2021. Induced autopolyploidy-A promising approach for enhanced biosynthesis of plant secondary metabolites: an insight. J Genet Eng Biotechnol 19 (1): 4. DOI:10.1186/s43141-020-00109-8.
Hailu MG, Mawcha KT, Nshimiyamana S, Suharsono S. 2021. Garlic micro-propagation and ploidy induction in vitro by colchicine. Plant Breed Biotechnol 9 (1): 1-19. DOI: 10.9787/PBB.2021.9.1.1.
Harrison JSH, Schwarzacher T, Liu Q. 2023. Polyploidy: its consequences and enabling role in plant diversification and evolution. Ann Bot 131: 1-9. DOI: 10.1093/aob/mcac132.
Khursheed, Shahnawaz, Raina A, Parveen K, Khan S. 2019. Induced phenotypic diversity in the mutagenized populations of faba bean using physical and chemical mutagenesis. J Saudi Soc Agric Sci 18 (2): 113-119. DOI: 10.1016/j.jssas.2017.03.001.
Mahajan V, Devi A, Khar A, Lawande KE. 2015. Studies on mutagenesis in garlic using chemical mutagens to determine lethal dose (LD50) and create variability. Indian J Hortic 72 (2): 289-292. DOI: 10.5958/0974-0112.2015.00055.9.
Manzoor, Touqeer A, Bashir MA. 2019. Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants. J Plants 8 (7), 194. DOI: 10.3390/plants8070194, www.mdpi.com/journal/plants.
Parsons JL, Martin SL, James T. 2019. Polyploidization for the genetic improvement of Cannabis sativa. Front Plant Sci 10: 1- 12. DOI: 10.3389/fpls.2019.00476.
Rajiman S, Megawati, Adiwijaya IMP, Permata ND. 2022. Agronomic character of shallot varieties on differences in plant spacing in rice fields. Ziraa'ah 47 (3): 384-393. DOI: 10.31602/zmip.v47i3.8166.
Samadi N, Naghavi MR, Lopez NM, Alonso G, Shokrpour M. 2022. Morphological, molecular and phytochemical variations induced by colchicine and EMS chemical mutagens in Crocus sativus L. Food Chem Mol Sci 4: 100086. DOI: 10.1016/j.fochms.2022.100086.
Sari Y, Sobir, Syukur M, Dinarti D. 2019. Induction of polyploid TSS (true shallot seed) of trident variety shallots using colchicine. Indones J Hortic 10 (3): 145-153. DOI: 10.29244/jhi.10.3.145-153.
Tammu RM, Nuringtyas TR, Daryono BS. 2021. Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia. J Genet Eng Biotechnol 19 (31): 1-8. DOI: 10.1186/s43141-021-00131-4.
Touchell DH, Palmer IE, Ranney TG. 2020. In vitro ploidy manipulation for crop improvement. Front Plant Sci 11: 722-731. DOI: 10.3389/fpls.2020.0072.
UPOV. 2008. Onion, echalion; shallot; grey shallot, guidlines for conduct of tests for distinctness, uniformity and stability. International Union for The Protection of New Varieties of Plants, Geneva. https://www.upov.int/edocs/tgdocs/en/tg046.pdf.
Woo YC, Deepo M, Islam MM, Si N, Hong YK, Jeung SH, Chang KK, Mi YC, Ki BL. 2021. Induction of polyploidy in Cucumis melo "Chammel" and evaluation of morphological and cytogenetic changes. Hortic Sci Technol 24: 625-636, DOI: 10.7235/HORT.20210056.
Yan JY, Qin SS, Zhou NZ, Xie Y, He Y. 2022. Effects of colchicine on polyploidy induction of Buddleja lindleyana seeds. Plant Cell Tissue Organ Cult (PCTOC) 149: 735-745. DOI: 10.1007/s11240-022-02245-0.

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