Variability of agromorphological traits in Portulaca grandiflora through induced mutation using colchicine
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Aisyah SI, Meiningrum NI, Yudha YS, Nurcholis W. 2024. Variability of agromorphological traits in Portulaca grandiflora through induced mutation using colchicine. Biodiversitas 25: 2484-2493. Portulaca grandiflora Hook is an ornamental plant originating from the Portulacaceae family, and it has been extensively cultivated on various continents. P. grandiflora displays appealing flower shapes and vibrant colors suitable for growth in a tropical landscape. Increased morphological diversity carries significant importance in the aesthetic function of ornamental plants. The higher the variation in the morphological characteristics of ornamental plants, the greater the market value of these plants due to their enhanced aesthetic appeal. Additionally, colchicine, a polyploidy-inducing agent, can enhance the plant genetic diversity. This study aims to investigate the impact of colchicine application on the morphological characters of P. grandiflora. The experiment was conducted for four months at the Leuwikopo Experimental Field, IPB, with two factors of Completely Randomized Block Design: The colchicine treatment comprises five concentration levels (0, 0.05, 0.1, 0.15, and 0.2), with four test genotypes: Orange, Peach, Bicolor, and Banana. A significant reduction in growth was observed in the Bicolor genotype, followed by the Banana genotype, indicating varying tissue sensitivities to colchicine treatment. The study reveals variations in morphological responses for each concentration treatment and genotype used. Statistical results confirm aspects: (i) each genotype displays different performance, (ii) the combined treatment of colchicine significantly influences the growth and development of quantitative traits, and (iii) The interaction (colchicine x genotype) was statistically significant for all quantitative characters of P. grandiflora except stem diameter. Qualitative traits, such as morphological changes in stem color, leaf color, and shape, were observed. Three correlation values are positive and statistically significant: plant length and leaf width, number of branches on shoots and leaves, and number of branches on shoots and flowers. Putative mutants of P. grandiflora exhibiting new traits require in-depth exploration in subsequent generations.
##plugins.themes.bootstrap3.article.details##
References
Aisyah SI, Saraswati RAM, Yudha YS, Nurcholis W. 2022. The diversity of agromorphological characters of Portulaca grandiflora in the MV8 population deriving from recurrent irradiation. Biodiversitas. 23(9):4432–4439.DOI:10.13057/biodiv/d230908.
Ajayi AT, Ohunakin AO, Osekita OS, Oki OC. 2014. Influence of Colchicine Treatments on Character Expression and Yield Traits in Cowpea (Vigna Unguiculata L. Walp). Glob. Journals Sci. Front. Res. C Biol. Sci 14(5):15–20.
Arindyaswari A, Etikawati N, Suratman. 2022. Effect of colchicine on chromosome number, morphological character and ß-carotene production of Amaranthus tricolor’s red giti cultivar. Cell Biol. Dev. 5(1):18–24.DOI:10.13057/cellbioldev/v050103.
Cabahug RAM, Ha MKTT, Lim KB, Hwang YJ. 2020. LD50 determination and phenotypic evaluation of three Echeveria varieties induced by chemical mutagens. Toxicol. Environ. Health Sci 12(1).DOI:10.1007/s13530-020-00049-3.
Chaudhary J, Deshmukh R, Sonah H. 2019. Mutagenesis approaches and their role in crop improvement. Plants 8(11):10–13.DOI:10.3390/plants8110467.
Cruz CF, dos Santos WF, da Silva Souza C, Machado MD, de Carvalho IF, Rocha DI, da Silva ML. 2019. In vitro regeneration and flowering of Portulaca grandiflora Hook. Ornam. Hortic 25(4):443–449. DOI:10.1590/2447-536X.v25i4.2077.
Eng WH, Ho WS. 2019. Polyploidization using colchicine in horticultural plants: A review. Sci. Hortic. (Amsterdam. 246:604–617.DOI:10.1016/j.scienta.2018.11.010.
Eng WH, Ho WS, Ling KH. 2021. Effects of colchicine treatment on morphological variations of Neolamarckia cadamba. Int. J. Agric. Techno. 17(1):47–66.
Fathurrahman F, Mardaleni, Krisianto A. 2023. Effect of colchicine mutagen on phenotype and genotype of Vigna unguiculata var. sesquipedalis the 7th generation. Biodiversitas 24(3):1408–1416.DOI:10.13057/biodiv/d240310.
Grad WE, Gomaa SE. 2020. Polypolidy and sweetener induction in different Stevia varieties using colchicine. Egypt. J. Plant Bree. 24(3):697–715.
Husnawati, Purwanto UMS, Rispriandari AA. 2020. Differences in parts of the purslane plant (Portulaca grandiflora Hook) in relation to total phenolic and flavonoid contents, and antioxidant activity. Curr. Biochem 7(1):10–20.
Le KC, Ho TT, Lee J Du, Paek KY, Park SY. 2020. Colchicine mutagenesis from long-term cultured adventitious roots increases biomass and ginsenoside production in wild ginseng (Panax ginseng Mayer). Agronomy 10(6):1–16. DOI:10.3390/agronomy10060785.
Mangena P. 2023. Impact of polyploidy induction for salinity stress mitigation in soybean (Glycine max L. Merrill). Plants 12(6).doi:10.3390/plants12061356.
Mangena P, Mushadu PN. 2023. Colchicine-induced polyploidy in leguminous crops enhances morpho-physiological characteristics for drought stress tolerance. Life 13(10):1966. DOI:10.3390/life13101966.
Manzoor A, Ahmad T, Bashir MA, Hafiz IA, Silvestri C. 2019. Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants. Plants 8(7):1–16. DOI:10.3390/plants8070194.
Mo L, Chen J, Lou X, Xu Q, Dong R, Tong Z, Huang H, Lin E. 2020. Colchicine-induced polyploidy in Rhododendron fortunei lindl. Plants 9(4). DOI:10.3390/plants9040424.
Mohammadi V, Talebi S, Ahmadnasab M, Mollahassanzadeh H. 2023. The effect of induced polyploidy on phytochemistry, cellular organelles and the expression of genes involved in thymol and carvacrol biosynthetic pathway in thyme (Thymus vulgaris). Front. Plant Sci 14(9):1–10. DOI:10.3389/fpls.2023.1228844.
Nandhini R, Aruna P, Sankari A, Ravikesavan R. 2019. Assessment of colchicine sensitivity in african marigold (Tagetes erecta) var. Pusa Narangi Gainda. Electron. J. Plant Breed 10(2):922–929. DOI:10.5958/0975-928X.2019.00118.2.
Narukulla V, Lahane Y, Fiske K, Pandey S, Ziogas V. 2023. Induction of polyploidy in citrus rootstocks through in vitro colchicine treatment of seed-derived explants. Agronomy 13(6):1–15. DOI:10.3390/agronomy13061442.
Niazian M, Nalousi AM. 2020. Artificial polyploidy induction for improvement of ornamental and medicinal plants. Plant Cell. Tissue Organ Cult 142(3):447–469. DOI:10.1007/s11240-020-01888-1.
Nurcholis W, Aisyah SI, Saraswati RAM, Yudha YS. 2023. Total phenolic, flavonoid contents, and antioxidant activity of three selected Portulaca grandiflora mutants in MV8 generation as a result of recurrent irradiation technique. J. Appl. Biol. Biotechnol 11(3):245–249. DOI:10.7324/JABB.2023.111110.
Pan IC, Lu YF, Wen PJ, Chen YM. 2019. Using colchicine to create Poinsettia (Euphorbia pulcherrima 3 Euphorbia cornastra) mutants with various morphological traits. HortScience 54(10):1667–1672. DOI:10.21273/HORTSCI14143-19.
Patel A, Miles A, Strackhouse T, Cook L, Leng S, Patel S, Klinger K, Rudrabhatla S, Potlakayala SD. 2023. Methods of crop improvement and applications towards fortifying food security. Front. Genome Ed 5(7):1–22. DOI:10.3389/fgeed.2023.1171969.
Petersen KK, Hagberg P, Kristiansen K. 2003. Colchicine and oryzalin mediated chromosome doubling in different genotypes of Miscanthus sinensis. Plant Cell Tissue Organ Cult 73(2):137–146. DOI:10.1023/A:1022854303371.
Pliankong P, Suksa-Ard P, Wannakrairoj S. 2017. Effects of colchicine and oryzalin on polyploidy induction and production of capsaicin in Capsicum frutescens L. Thai J. Agric. Sci 50(2):108–120.
Rasheed N, Ahmad I, Nafees M, Intizar-Ul-Hassan M. 2022. Assessment of mutation caused by colchicine in apple gourd (Praecitrullus fistulosus) based on morphological and biochemical attributes. J. Glob. Innov. Agric. Sci 10(1):19–24. DOI:10.22194/JGIAS/10.972.
Roy S, Thakur A, Singh R, Kumar A, Yadav S. 2023. Determination of semi-lethal dose of colchicine on in vitro grown callus of Azadirachta indica genotypes. Appl. Biol. Chem. J (5):28–33. DOI:10.52679/tabcj.2023.0003.
Samatadze TE, Yurkevich OY, Khazieva FM, Basalaeva I V., Konyaeva EA, Burova AE, Zoshchuk SA, Morozov AI, Amosova A V., Muravenko O V. 2022. Agro-morphological and cytogenetic characterization of colchicine-induced tetraploid plants of Polemonium caeruleum L. (Polemoniaceae). Plants 11(19). DOI:10.3390/plants11192585.
Sari B, Karno K, Anwar S. 2017. Cytological and morphological characteristics of polyploid moss rose (Portulaca grandiflora) resulted by application of colchicine treatment at different concentrations and frequencies. J. Agro Complex 1(2):39–48. DOI:10.14710/joac.1.2.39-48.
Sari DN, Aisyah SI, Damanik DMRM. 2017. Sensitivity and performance of Coleus sp. by chemical mutation induction using ethyl methane sulfonate (EMS) applied by soaking and dripping. Indones. J. Agron 45(1):56–63. DOI:10.24831/jai.v45i1.13157.
Setiawan FID, Aisyah SI, Krisantini K. 2016. Characterization of 13 accessions of purslane (Portulaca sp.) from Bogor, West Java, Indonesia. J. Trop. Crop Sci 3(3):67–74. DOI:10.29244/jtcs.3.3.67-74.
Sinay H, Tanrobak J. 2020. Heritability analysis of local corn cultivars from kisar island southwest maluku after induced with colchicine. Biosaintifika 12(1):119–124. DOI:10.15294/biosaintifika.v12i1.19847.
Sodiq NAM, Fathurrahman F, Ulpah S. 2023. The influence of colchicine concentration on the growth and production of black soybean variety detam 2 (Glycine soja (L.) Merr). Ekoagrotrop 2(1):1–9.
Susrama IGK, Trigunasih NM, Suada IK, Mayadewi NNA. 2022. Performance evaluation of induced mutagenesis using colchicine and EMS solution on cowpea M3 purple and mung bean Vima1 to Increase Resistance. Caraka Tani J. Sustain. Agric 37(2):333–343. DOI:10.20961/carakatani.v37i2.56341.
Tossi VE, Martínez Tosar LJ, Laino LE, Iannicelli J, Regalado JJ, Escandón AS, Baroli I, Causin HF, Pitta-Álvarez SI. 2022. Impact of polyploidy on plant tolerance to abiotic and biotic stresses. Front. Plant Sci 13(8):1–19. DOI:10.3389/fpls.2022.869423.
Touchell DH, Palmer IE, Ranney TG. 2020. In vitro Ploidy Manipulation for Crop Improvement. Front. Plant Sci 11(6):1–11. DOI:10.3389/fpls.2020.00722.
Zhang YS, Chen JJ, Cao YM, Duan JX, Cai XD. 2020. Induction of tetraploids in ‘Red Flash’ caladium using colchicine and oryzalin: Morphological, cytological, photosynthetic and chilling tolerance analysis. Sci. Hortic. (Amsterdam) 272(4):109524. DOI:10.1016/j.scienta.2020.109524.
Most read articles by the same author(s)
- WARAS NURCHOLIS, NURUL KHUMAIDA, MARIA BINTANG, MUHAMAD SYUKUR, GC-MS analysis of rhizome ethanol extracts from Curcuma aeruginosa accessions and their efficiency activities as anticancer agent , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 3 (2021)
- JANSON CALVINDI, MUHAMAD SYUKUR, WARAS NURCHOLIS, Investigation of biochemical characters and antioxidant properties of different winged bean (Psophocarpus tetragonolobus) genotypes grown in Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 6 (2020)
- NURUL KHUMAIDA, MUHAMAD SYUKUR, MARIA BINTANG, WARAS NURCHOLIS, Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 3 (2019)
- RIZKI FITRIA, DJAROT SASONGKO HAMI SENO, BAMBANG PONTJO PRIOSOERYANTO, HARTANTI, WARAS NURCHOLIS, Volatile compound profiles and cytotoxicity in essential oils from rhizome of Curcuma aeruginosa and Curcuma zanthorrhiza , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 10 (2019)
- ZULFIKAR DAMARALAM SAHID, MUHAMAD SYUKUR, AWANG MAHARIJAYA, WARAS NURCHOLIS, Quantitative and qualitative diversity of chili (Capsicum spp.) genotypes , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 2 (2022)
- SYARIFAH IIS AISYAH, IZZATUL MUHALLILIN, DEWI SUKMA, WARAS NURCHOLIS, The morphological and phytochemical studies on the effect of acute and recurrent irradiation in Celosia cristata seeds , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 12 (2019)
- SURYANI, ASMAUL CHUSNA AL ANSHORY, MARLIN, I MADE ARTIKA, LAKSMI AMBARSARI, WARAS NURCHOLIS, Variability total phenolic content and antioxidant activity of Curcuma zanthorrhiza and C. aeruginosa cultivated in three different locations in West Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 4 (2022)
- DEWI YANNY LENAWATY, DEWI SUKMA, MUHAMAD SYUKUR, DEWA NGURAH SUPRAPTA, WARAS NURCHOLIS, SYARIFAH IIS AISYAH, Increasing the diversity of marigold (Tagetes sp.) by acute and chronic chemical induced mutation of EMS (Ethyl Methane Sulfonate) , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 3 (2022)
- NURLELA NURLELA, RIFAN NURFALAH, FIRA ANANDA, TAOPIK RIDWAN, AULIYA ILMIAWATI, WARAS NURCHOLIS, HIROSHI TAKEMORI, IRMANIDA BATUBARA, Variation of morphological characteristics, total phenolic, and total flavonoid in Adenostemma lavenia, A. madurense, and A. platyphyllum , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 8 (2022)